High-Throughput Plasma FIB-SEM for Advanced Life Sciences Research
Product Overview
The TESCAN AMBER X LS is a state-of-the-art plasma-focused ion beam scanning electron microscope (FIB-SEM) tailored for life sciences applications. Featuring the advanced iFIB+ column, it enables rapid material removal and sample thinning, significantly enhancing throughput for cryo-electron tomography (cryo-ET) sample preparation. This system excels in handling both soft and hard biological specimens, including tissues, bones, and shells, making it indispensable for core facilities and imaging centers.
Key Features
- Ultra-Fast Cryo-ET Sample Preparation: The iFIB+ plasma FIB column accelerates material removal and sample thinning, addressing major bottlenecks in cryo-ET workflows.
- Efficient Cryo On-Grid Lamella Preparation: High-performance plasma FIB and optimized workflows enable rapid on-grid lamella preparation, essential for high-throughput cryo-EM sample processing.
- Intuitive Cryo Lift-Out: The TESCAN cryo-nanomanipulator facilitates easy cryo lift-out with precise movement and intuitive probe tip navigation.
- Versatile Sample Processing: Capable of handling soft and hard specimens, as well as those with mixed compositions, without introducing curtaining artifacts, thanks to TESCAN’s True X-Sectioning kit and Rocking Stage.
- Enhanced Cryo-Microscopy Productivity: Seamless integration with Leica Microsystems GmbH and Quorum Technologies Ltd cryo systems ensures convenient and safe microscope operation under cryogenic conditions.
- Safe Navigation: A unique 3D chamber model provides collision protection, allowing quick and safe navigation to regions of interest.
- User-Friendly Software: TESCAN Essence™ software offers ready-to-use, guided, and customizable workflow templates, enabling new users to become productive quickly while allowing experts to customize microscope controls.
Specifications
| Feature | Specification |
|---|---|
| Ion Source | Xenon Plasma FIB (iFIB+ column) |
| Electron Source | High-Brightness Schottky Field Emission Gun (FEG) |
| Imaging Resolution | High-resolution SEM imaging capabilities |
| Cryo Compatibility | Supports cryo-FIB and cryo-SEM workflows |
| Sample Handling | Accommodates soft and hard biological specimens, including mixed compositions |
| Software | TESCAN Essence™ with guided and customizable workflow templates |
| Integrated Cryo Systems | Compatible with Leica Microsystems GmbH and Quorum Technologies Ltd cryo systems for seamless operation |
| Artifact Prevention | TESCAN’s proprietary methods to prevent curtaining artifacts during milling |
| Navigation | 3D chamber model for collision protection and efficient navigation |
| Field of View | Exceptional FIB field of view covering up to 1 mm for cross-sectioning and large-volume analyses |
Applications
- Cryo-Electron Tomography (Cryo-ET): Rapid and precise preparation of on-grid lamellae from plunge-frozen specimens, enhancing the efficiency of cryo-ET workflows.
- 3D FIB-SEM Tomography: High-resolution 3D reconstruction of large volumes, suitable for hard biological materials such as bones and shells.
- Biomedical Research: Detailed structural analysis of tissues and cells, facilitating studies in various biomedical fields.
- Materials Science: Versatile capabilities make it suitable for analyzing a variety of materials and specimens, both at ambient and cryogenic temperatures.
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