High-Throughput Quantitative Single-Molecule Force Spectroscopy
Product Overview
The Bruker JPK ForceRobot 400 is a state-of-the-art BioAFM system designed for high-throughput single-molecule force spectroscopy (SMFS). It enables researchers to probe the mechanical properties of molecular bonds, materials, and biomolecular complexes with nanometer precision and piconewton sensitivity.
Combining fully automated workflows, next-generation analysis, and seamless integration with advanced optical techniques, ForceRobot 400 redefines performance in single-molecule nanomechanics. From understanding receptor-ligand interactions to characterizing mechanical behavior in semiconductor interfaces, the ForceRobot 400 provides unmatched speed, accuracy, and flexibility in nanoscale force measurements.
Key Features
- Collect up to 250,000 force curves per day and more than 10,000 per hour
- Fully automated SMFS with advanced force curve fitting and statistical analysis
- SmartMapping for flexible ROI selection and automated z-positioning
- NestedScanner option for high-frequency microrheology measurements (≥5 kHz)
- Real-time auto-alignment, calibration, and adaptive environmental tuning
- Modular software with ExperimentPlanner and long-term unattended workflows
- Seamless integration with optical microscopy platforms (fluorescence, DIC, TIRF, super-resolution)
- Supports a wide range of force spectroscopy modes: ramp, clamp, microrheology
- Environmentally controlled sample chamber with humidity, temperature, and gas flow regulation
Specifications
| Specification | Details |
|---|---|
| Max Force Curve Acquisition | >250,000 curves/day, >10,000/hour |
| Vertical Travel Range | >19 mm (combined piezo + motor) |
| Z-Scanner (Optional) | 15 µm capacitive scanner with closed-loop control (SLD 880 or 980 nm) |
| Z-Piezo Option | Additional 1.5 µm range |
| Software Platform | SPMControl V8 with ExperimentPlanner, auto-calibration, SmartMapping |
| Stage Travel (Motorized) | 20 × 20 mm² |
| Precision Mapping Stage | 100 × 100 µm² piezo range |
| Sample Compatibility | Petri dishes, coverslips, slides, metal discs |
| Optical Microscope Integration | Compatible with Nikon, Zeiss, Olympus, Leica (inverted/upright) |
| Optical Modes Supported | Brightfield, phase contrast, DIC, CLSM, STED, STORM, FRAP, TIRF |
| Environmental Control | Temp (up to 300°C), humidity, perfusion, CO₂, ionic strength, fluid flow |
| Optional Add-Ons | FluidFM, SmallCell, Stretching Stage, SideView Holder, DirectTiling |
| Controller | Vortis® 2.1 SPM Controller |
| Operating Modes | Contact Mode, Advanced Force Mapping, Microrheology, Force Clamp/Ramp |
Applications
Academia & Research Institutions
- Single-molecule unfolding, binding/unbinding studies
- Quantification of molecular biomechanics and elasticity
- Development of next-gen therapeutic molecules via force-probing
Semiconductor & Electronics
- Measurement of viscoelastic and adhesive properties of thin films
- Analysis of nanoscale bond strength in heterostructures
- Force mapping of functionalized surfaces and polymers under varied environments
Electrical & Electronic Materials
- Characterization of nanomechanical behavior of dielectric layers and conductive polymers
- Adhesion and mechanical stress testing in MEMS/NEMS and printed electronics
- In-situ testing of hybrid materials (e.g., graphene/polymer composites)
Resources
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